Tecnobios

Pubblicazioni

A quantitative approach to blastocyst quality evaluation: morphometric analysis and related IVF outcomes.

Lagalla C, Barberi M, Orlando G, Sciajno R, Bonu MA, Borini A.

J Assist Reprod Genet. 2015 May;32(5):705-12. doi: 10.1007/s10815-015-0469-3. Epub 2015 Apr 9.

PMID: 25854656

Abstract

PURPOSE:

To quantify blastocyst morphologic parameters with a feasible and standardized tool, investigating their predictive value on implantation outcome.

METHOD:

The study retrospectively analyzes 124 blastocysts from 75 patients. Quantitative measurements of blastocyst expansion, inner cell mass and trophoectoderm were taken using digital image analysis software.

RESULT(S):

Blastocysts areas were found to be ranging from 11626.2 up to 35076.4 μm(2). The area of an early blastocyst is A ≤ 18500 μm(2) with a mean diameter d = 140 ± 9 μm, and the area of an expanded blastocyst is A ≥ 24000 with d = 190 ± 9 μm. While blastocyst mean area was not related to implantation rate, more expanded blastocysts displayed a significantly higher implantation rate. Trophoectoderm cell number is a predictor of positive outcome: since a higher of cells (25.6 ± 11.3 vs 16.3 ± 12.8) `forming a tightly knit epithelium is prognostic of implantation potential. Conversely, inner cell mass size is significantly related to implantation only in expanded blastocysts (3122.7 ± 739.0 vs. 2978.1 ± 366.0 μm(2)).

CONCLUSION(S):

Evaluation of blastocyst morphology with a digital image system could be a valuable tool to standardize blastocyst grading based on quantitative parameters. Therefore, digital analysis may be helpful in identifying the best blastocyst to transfer.


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